FilmArray Respiratory Panel Assay: Comparison of Nasopharyngeal Swabs and Bronchoalveolar Lavage Samples
| Autor: | Kenneth K. Sakata, Natalya Azadeh, Holenarasipur R. Vikram, Thomas E. Grys, Anjuli Brighton |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Male Polymerase Chain Reaction Sensitivity and Specificity law.invention Specimen Handling law Virology Nasopharynx Medicine Molecular diagnostic techniques Humans Respiratory system Pathogen Respiratory Tract Infections Polymerase chain reaction Retrospective Studies medicine.diagnostic_test Respiratory tract infections business.industry respiratory system Middle Aged respiratory tract diseases Bronchoalveolar lavage Molecular Diagnostic Techniques Immunology Female business Bronchoalveolar Lavage Fluid Multiplex Polymerase Chain Reaction |
| Zdroj: | Journal of Clinical Microbiology |
| Popis: | The FilmArray respiratory panel (FARP) reliably and rapidly identifies 17 viruses and 3 bacterial pathogens. A nasopharyngeal swab FARP (NP FARP) is performed for many patients with respiratory symptoms. For patients who are acutely ill or immunocompromised or fail to improve, a bronchoalveolar lavage sample FARP (BAL FARP) is performed in addition to the NP FARP. To date, no studies have compared the yield of a BAL FARP with that of an NP FARP. We retrospectively studied all patients who had a BAL FARP within 7 days after an NP FARP between June 2013 and May 2014. Demographic information, comorbidities, FARP results, and all microbiologic data from BAL fluid were collected. Eighty-six patients had a BAL FARP performed within 7 days (mean, 1.6; median, 1) after an NP FARP. Of these, 66 (77%) had concordant BAL and NP FARP results: 15 (23%) had the same pathogen identified from the NP and BAL FARPs, and 51 (77%) had concordant negative FARP results. In 18 of the 86 patients (21%), a pathogen was detected from the NP FARP; of these, 15 (83%) had a concordant match on a subsequent BAL FARP, and the remaining 3 had negative BAL FARPs. In 17 of the 86 patients (20%), pathogens were identified from the BAL FARPs that were not detected by the NP FARPs; of these, 16 (94%) had initial negative NP FARPs. The data suggest that once a pathogen is identified by an NP FARP, a subsequent BAL FARP is unlikely to add new microbiologic information. However, a BAL FARP may provide new, useful microbiologic information when performed within 7 days after a negative NP FARP. |
| Databáze: | OpenAIRE |
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