The Anti-Inflammatory Effect of Hydrogen Gas Inhalation and Its Influence on Laser-Induced Choroidal Neovascularization in a Mouse Model of Neovascular Age-Related Macular Degeneration

Autor: Pei-Chen Lai, Chi-Feng Hung, Xv-Hui Zong, Wen-Chin Ko, Yun-Hsiang Chang, I-Chia Liang, Der-Chen Chang, Yi-Ru Lin, Yu-Jou Hsu
Jazyk: angličtina
Rok vydání: 2021
Předmět:
genetic structures
Anti-Inflammatory Agents
Angiogenesis Inhibitors
Pharmacology
medicine.disease_cause
Macular Degeneration
Mice
chemistry.chemical_compound
Medicine
hydrogen gas
Fluorescein Angiography
Biology (General)
Spectroscopy
Inhalation
General Medicine
Computer Science Applications
Vascular endothelial growth factor
Chemistry
Choroidal neovascularization
Tumor necrosis factor alpha
Gases
medicine.symptom
Fundus Oculi
QH301-705.5
Alpha (ethology)
Inflammation
choroidal neovascularization
Catalysis
Article
Inorganic Chemistry
Administration
Inhalation
Animals
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
age-related macular degeneration
business.industry
Lasers
Organic Chemistry
Macular degeneration
medicine.disease
eye diseases
Mice
Inbred C57BL
Disease Models
Animal
chemistry
Wet Macular Degeneration
sense organs
business
Oxidative stress
Hydrogen
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 21
International Journal of Molecular Sciences, Vol 22, Iss 12049, p 12049 (2021)
ISSN: 1422-0067
Popis: Background: Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly. Choroidal neovascularization (CNV) is the major pathologic feature of neovascular AMD. Oxidative damages and the ensuing chronic inflammation are representative of trigger events. Hydrogen gas (H2) has been demonstrated as an antioxidant and plays a role in the regulation of oxidative stress and inflammation. This experiment aimed to investigate the influence of H2 inhalation on a mouse model of CNV. Methods: Laser was used to induce CNV formation. C57BL/6J mice were divided into five groups: the control group
the laser-only group
and the 2 h, 5 h, and 2.5 h/2.5 h groups that received laser and H2 inhalation (21% oxygen, 42% hydrogen, and 37% nitrogen mixture) for 2 h, 5 h, and 2.5 h twice every day, respectively. Results: The severity of CNV leakage on fluorescence angiography showed a significant decrease in the H2 inhalation groups. The mRNA expression of hypoxia-inducible factor 1 alpha and its immediate downstream target vascular endothelial growth factor (VEGF) showed significant elevation after laser, and this elevation was suppressed in the H2 inhalation groups in an inhalation period length-related manner. The mRNA expression of cytokines, including tumor necrosis factor alpha and interlukin-6, also represented similar results. Conclusion: H2 inhalation could alleviate CNV leakage in a laser-induced mouse CNV model, and the potential mechanism might be related to the suppression of the inflammatory process and VEGF-driven CNV formation.
Databáze: OpenAIRE
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