Cytotoxic ellagitannins from Reaumuria vermiculata
| Autor: | Mohamed A. El-Rai, Kristian Wende, Mahmoud A.M. Nawwar, Ahmed M. Al-Abd, Manuela Harms, Fatma Bassyouny, Eman S. Mostafa, Michael W. Linscheid, Nahla A. Ayoub, Ulrike Lindequist |
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| Rok vydání: | 2012 |
| Předmět: |
Keratinocytes
Male Antioxidant Oxygen radical absorbance capacity medicine.medical_treatment Cell Antioxidants In vivo Cell Line Tumor Neoplasms Drug Discovery medicine Humans Cytotoxicity Pharmacology Molecular Structure Plant Extracts Tamaricaceae Chemistry Carcinoma General Medicine Plant Components Aerial Antineoplastic Agents Phytogenic Hydrolyzable Tannins HaCaT medicine.anatomical_structure Biochemistry Cell culture Female Two-dimensional nuclear magnetic resonance spectroscopy Phytotherapy |
| Zdroj: | Fitoterapia. 83:1256-1266 |
| ISSN: | 0367-326X |
| DOI: | 10.1016/j.fitote.2012.06.007 |
| Popis: | Three ellagitannins and one disulfated flavonol were isolated from the aerial parts of Reaumuria vermiculata L. Besides that, 16 known compounds were characterized as well. The structures of all compounds were elucidated on the basis of spectroscopic data including 1D and 2D NMR and ESI HR-FTMS. The in vivo antioxidant activity using the oxygen radical absorbance capacity (ORAC) method, of the extract, its column fractions and two of the isolated ellagitannins was accomplished. In addition, a possible cytotoxicity of the extract and two of the new ellagitannins on HaCaT human keratinocytes and the activity of both compounds against the prostate cancer cell line (PC-3) were also assessed, whereby a potent cytotoxicity with IC 50 less than 1 μg/ml was determined for both compounds. Besides, the extract exhibited a potential cytotoxic effect against four different solid tumor cell lines, namely liver (Huh-7), colorectal (HCT-116), breast (MCF-7) and prostate (PC-3). The IC 50 s were found to be substantially low (ranged from 1.3 ± 0.15 to 2.4 ± 0.22 μg/ml) with relatively low resistance possibility reaching to 0% in the case of Huh-7 cell. |
| Databáze: | OpenAIRE |
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