Moderate Alcohol Consumption Uniquely Regulates Sodium-Dependent Glucose Co-Transport in Rat Intestinal Epithelial Cells In Vitro and In Vivo
| Autor: | Soudamani Singh, Uma Sundaram, Subha Arthur, Jennifer Haynes, Molly R. Butts |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Brush border Sodium Medicine (miscellaneous) chemistry.chemical_element Intestinal absorption Cell Line Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Sodium-Glucose Transporter 1 0302 clinical medicine Western blot In vivo Internal medicine Intestine Small medicine Animals Intestinal Mucosa Nutrition and Dietetics Ethanol SLC5A1 Microvilli biology medicine.diagnostic_test Epithelial Cells In vitro Rats Glucose 030104 developmental biology Endocrinology Intestinal Absorption chemistry biology.protein Nutrient Physiology Metabolism and Nutrient-Nutrient Interactions Sodium-Potassium-Exchanging ATPase 030217 neurology & neurosurgery |
| Zdroj: | J Nutr |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/nxz277 |
| Popis: | BACKGROUND: Chronic alcohol use often leads to malnutrition. However, how the intestinal absorption of nutrients such as glucose may be affected during moderate ethanol use has not been investigated. Glucose is absorbed via sodium (Na)-dependent glucose co-transport (SGLT1; SLC5A1) along the brush border membrane (BBM) of intestinal absorptive villus cells. OBJECTIVE: The aim of this study was to investigate how moderate alcohol consumption affects the absorption of glucose via SGLT1. METHODS: Intestinal epithelial cells (IEC-18; rat) were exposed to 8.64 mM ethanol over 1, 3, 6, and 12 h. Rats (16-wk-old, male, Sprague-Dawley) were administered 2 g/kg ethanol over 1, 3, and 6 h. Na-dependent (3)H-O-methyl-d-glucose uptake was measured to assess SGLT1 activity. Na-K-ATPase activity was measured as a function of inorganic phosphate release. Protein expression was analyzed by Western blot analysis and immunohistochemical staining. RESULTS: Ethanol significantly decreased Na-dependent glucose absorption in enterocytes in vitro (ethanol treatment: 48.4% of controls at 1 h; P |
| Databáze: | OpenAIRE |
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