In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite
| Autor: | Maria Chatzinikolaidou, Rafaela-Maria Kavasi, Paulo A. Quadros, Varvara Platania, Catarina C. Coelho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
General Chemical Engineering media_common.quotation_subject Nanoparticle 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Article Dynamic light scattering cosmetics and care products medicine General Materials Science Cytotoxicity Internalization QD1-999 media_common toxicological and safety assessment Chemistry genotoxicity hydroxyapatite cellular uptake 021001 nanoscience & nanotechnology 0104 chemical sciences internalization micronucleus test Drug delivery Micronucleus test Biophysics cytotoxicity nanoparticles 0210 nano-technology Genotoxicity |
| Zdroj: | Nanomaterials Volume 11 Issue 5 Nanomaterials, Vol 11, Iss 1152, p 1152 (2021) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano11051152 |
| Popis: | Hydroxyapatite (HA) is an important component of the bone mineral phase. It has been used in several applications, such as bone regenerative medicine, tooth implants, drug delivery and oral care cosmetics. In the present study, three different batches of a commercial nanohydroxyapatite (nHA) material were physicochemically-characterized and biologically-evaluated by means of cytotoxicity and genotoxicity using appropriate cell lines based on well-established guidelines (ISO10993-5 and OECD 487). The nHAs were characterized for their size and morphology by dynamic light scattering (DLS) and transmission electron microscopy (TEM) and were found to have a rod-like shape with an average length of approximately 20 to 40 nm. The nanoparticles were cytocompatible according to ISO 10993-5, and the in vitro micronucleus assay showed no genotoxicity to cells. Internalization by MC3T3-E1 cells was observed by TEM images, with nHA identified only in the cytoplasm and extracellular space. This result also validates the genotoxicity since nHA was not observed in the nucleus. The internalization of nHA by the cells did not seem to affect normal cell behavior, since the results showed good biocompatibility of these nHA nanoparticles. Therefore, this work is a relevant contribution for the safety assessment of this nHA material. |
| Databáze: | OpenAIRE |
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