Suppression of inducible nitric oxide synthase mRNA expression by tryptoquinone A
| Autor: | Yumiko Kawai, Noriko Nakamura, Mineo Niwa, Shiroh Futaki, Yasuyuki Tsutsumishita, Yoshihisa Takaishi, Hideo Takahara, Hideki Moritoki, Wataru Kondoh |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Nitroprusside Time Factors Transcription Genetic Mrna expression Radical Muscle Relaxation Vasodilator Agents Molecular Sequence Data Biophysics Aorta Thoracic In Vitro Techniques Arginine Biochemistry Polymerase Chain Reaction Dexamethasone Gene Expression Regulation Enzymologic Muscle Smooth Vascular Nitric oxide chemistry.chemical_compound Phenylephrine medicine Animals RNA Messenger Rats Wistar Molecular Biology Cyclic GMP DNA Primers chemistry.chemical_classification Smooth muscle relaxation biology Base Sequence Anti-Inflammatory Agents Non-Steroidal Cell Biology Plants Molecular biology Rats Nitric oxide synthase Isoenzymes Kinetics Enzyme chemistry Inos mrna biology.protein Diterpenes Nitric Oxide Synthase medicine.drug Interleukin-1 |
| Zdroj: | Biochemical and biophysical research communications. 224(2) |
| ISSN: | 0006-291X |
| Popis: | Triptoquinone A (TQA), which is an anti-inflammatory constituent in plants, was studied for its suppressive effect on nitric oxide production by LPS. TQA significantly suppressed smooth muscle relaxation and increase in cyclic GMP levels by nitric oxide (NO) in an L-arginine-induced relaxation experiment. The mechanistic studies showed that TQA did not directly inhibit NO radicals and inducible nitric oxide synthase (iNOS) enzyme but suppressed IL-1 β and iNOS mRNA expression by LPS. The suppression level of iNOS gene expression by TQA was comparable to that by dexamethasone. TQA may be a useful candidate for the development of a drug as a potent inhibitor of iNOS gene over-expression. |
| Databáze: | OpenAIRE |
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