Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
| Autor: | Benjamin P. Berman, Linda Marbán, Luis R. Borlado, Geoffrey de Couto, Liang Li, Travis J. Antes, Tanner Quon, Eduardo Marbán, Kiel Peck, Sasha Smolgovsky, Ann-Sophie Walravens, Lauren Kelly, Ahmed Ibrahim |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Cell type RNA Untranslated Science Cell Myocardial Infarction Mice SCID 030204 cardiovascular system & hematology Biology Mesenchymal Stem Cell Transplantation Real-Time Polymerase Chain Reaction Article Cell therapy Extracellular Vesicles Mice Young Adult 03 medical and health sciences 0302 clinical medicine Immune system In vivo medicine Animals Humans Myocytes Cardiac Child Acute inflammation Monocytes and macrophages Multidisciplinary Mesenchymal stem cell Mesenchymal Stem Cells Extracellular vesicle Middle Aged Cell biology Mice Inbred C57BL Disease Models Animal Treatment Outcome Cardiovascular diseases 030104 developmental biology medicine.anatomical_structure Child Preschool Medicine Female Cardiac regeneration Heart stem cells Adult stem cell |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Cell therapy limits ischemic injury following myocardial infarction (MI) by preventing cell death, modulating the immune response, and promoting tissue regeneration. The therapeutic efficacy of cardiosphere-derived cells (CDCs) and mesenchymal stem cells (MSCs) is associated with extracellular vesicle (EV) release. Prior head-to-head comparisons have shown CDCs to be more effective than MSCs in MI models. Despite differences in cell origin, it is unclear why EVs from different adult stem cell populations elicit differences in therapeutic efficacy. Here, we compare EVs derived from multiple human MSC and CDC donors using diverse in vitro and in vivo assays. EV membrane protein and non-coding RNA composition are highly specific to the parent cell type; for example, miR-10b is enriched in MSC-EVs relative to CDC-EVs, while Y RNA fragments follow the opposite pattern. CDC-EVs enhance the Arg1/Nos2 ratio in macrophages in vitro and reduce MI size more than MSC-EVs and suppress inflammation during acute peritonitis in vivo. Thus, CDC-EVs are distinct from MSC-EVs, confer immunomodulation, and protect the host against ischemic myocardial injury and acute inflammation. |
| Databáze: | OpenAIRE |
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