Glucagon-like peptide-1 receptor expression in the human eye
| Autor: | Peter Helding Kvist, Josephine B Hebsgaard, Steffen Heegaard, Emre Yildirim, Charles Pyke, Lotte Bjerre Knudsen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Endocrinology Diabetes and Metabolism Receptor expression RNA Messenger/metabolism Eye Diabetic Retinopathy/etiology Glucagon-Like Peptide-1 Receptor/metabolism 0302 clinical medicine Endocrinology cardiovascular disease Receptors Glucagon Receptor In Situ Hybridization Brief Report digestive oral and skin physiology Diabetic retinopathy Middle Aged Immunohistochemistry diabetic retinopathy medicine.anatomical_structure Female hormones hormone substitutes and hormone antagonists Adult medicine.medical_specialty endocrine system 030209 endocrinology & metabolism In situ hybridization Glucagon-Like Peptide-1 Receptor 03 medical and health sciences GLP‐1 analogue Internal medicine Internal Medicine medicine Humans RNA Messenger Ganglion cell layer Eye/metabolism Aged Retina business.industry Semaglutide Diabetes Mellitus Type 2/complications diabetes complications medicine.disease Glucagon 030104 developmental biology Diabetes Mellitus Type 2 Brief Reports sense organs business |
| Zdroj: | Hebsgaard, J B, Pyke, C, Yildirim, E, Knudsen, L B, Heegaard, S & Kvist, P H 2018, ' Glucagon-like peptide-1 receptor expression in the human eye ', Diabetes, Obesity and Metabolism, vol. 20, no. 9, pp. 2304-2308 . https://doi.org/10.1111/dom.13339 Diabetes, Obesity & Metabolism |
| DOI: | 10.1111/dom.13339 |
| Popis: | Semaglutide is a human glucagon-like peptide-1 (GLP-1) analogue that is in development for the treatment of type 2 diabetes. In the pre-approval cardiovascular outcomes trial SUSTAIN 6, semaglutide was associated with a significant increase in the risk of diabetic retinopathy (DR) complications vs placebo. GLP-1 receptor (GLP-1R) expression has previously been demonstrated in the retina in animals and humans; however, antibodies used to detect expression have been documented to be non-specific and fail to detect the GLP-1R using immunohistochemistry (IHC), a problem common for many G-protein coupled receptors. Using a validated GLP-1R antibody for IHC and in situ hybridization for GLP-1R mRNA in normal human eyes, GLP-1Rs were detected in a small fraction of neurons in the ganglion cell layer. In advanced stages of DR, GLP-1R expression was not detected at the protein or mRNA level. Specifically, no GLP-1R expression was found in the eyes of people with long-standing proliferative DR (PDR). In conclusion, GLP-1R expression is low in normal human eyes and was not detected in eyes exhibiting advanced stages of PDR. |
| Databáze: | OpenAIRE |
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