Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells
| Autor: | Francisco J. de Toro, Silvia Díaz-Prado, Isaac Fuentes-Boquete, R. Castro-Viñuelas, Francisco J. Blanco, Tamara Hermida-Gómez, Clara Sanjurjo-Rodríguez |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Hyaline cartilage Mesenchymal stromal cells Osteoarthritis Matrix (biology) 03 medical and health sciences Medicine General & Internal Tissue engineering General & Internal Medicine medicine Tissue scaffolds Science & Technology Chemistry Cartilage Mesenchymal stem cell General Medicine medicine.disease Chondrogenesis 030104 developmental biology medicine.anatomical_structure Regenerative medicine Fibrocartilage Life Sciences & Biomedicine |
| Zdroj: | RUC. Repositorio da Universidade da Coruña instname |
| Popis: | [Abstract] The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 24 h; also, samples were not pre-treated. hBMSCs were seeded on different types of collagen scaffolds. The resulting constructs were placed into the lesions, and the biopsies were cultured for 2 months in chondrogenic medium. Using the modified ICRSII scale, neotissues from the different scaffolds showed ICRS II overall assessment scores ranging from 50% (fibrocartilage) to 100% (hyaline cartilage), except for the Col I +Col II +HS constructs (fibrocartilage/hyaline cartilage, 73%). Data showed that hBMSCs cultured only on Col I +Col II +HS scaffolds displayed a chondrocyte-like morphology and cartilage-like matrix close to native cartilage. Furthermore, IL1β pre-treated biopsies decreased capacity for repair by hBMSCs and decreased levels of chondrogenic phenotype of human cartilage lesions. Instituto de Salud Carlos III; CB06/01/0040 Xunta de Galicia ; R2016/036 Xunta de Galicia; R2014/050 Xunta de Galicia; GPC2014/048 Ministerio de Esconomía, Industria y Competitividad; RTC-2016-5386-1 Madrid (Comunidad Autónoma); S2009/MAT-1472 |
| Databáze: | OpenAIRE |
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