16S rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae
| Autor: | Kouichi Ohwada, Kumiko Kita-Tsukamoto, Hidetoshi Urakawa |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Genotype Molecular Sequence Data Vibrionaceae DNA Ribosomal Polymerase Chain Reaction Microbiology law.invention Endonuclease law RNA Ribosomal 16S Genetics Molecular Biology Genotyping Polymerase chain reaction Base Sequence biology biology.organism_classification 16S ribosomal RNA Restriction enzyme biology.protein Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | FEMS Microbiology Letters. 152:125-132 |
| ISSN: | 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1997.tb10418.x |
| Popis: | The 16S rDNA genotypes among the family Vibrionaceae were determined using PCR/RFLP analysis. Five tetrameric restriction enzymes (HhaI, DdeI, RsaI, Sau3AI and MspI) were used for RFLP analysis and adequate numbers of informative bands were obtained from each enzyme. Twenty-seven genotypes were obtained from 49 type and reference strains including 35 species. Nineteen species could be assigned to specific 16S rDNA genotypes, supporting the application of this analysis for identification. Trees constructed using five endonucleases resolved groups almost identical to those inferred from 16S rRNA gene sequencing. However, the branch lengths and detailed relationships among strains within a group differed from those inferred from sequence comparisons. The results of this study should be useful for genotyping, identification and approximate classification of natural isolates belonging to the family Vibrionaceae. |
| Databáze: | OpenAIRE |
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