Chemotherapeutic Effect of CD147 Antibody-labeled Micelles Encapsulating Doxorubicin Conjugate Targeting CD147-Expressing Carcinoma Cells
| Autor: | Tadashi Asakura, Masayuki Yokoyama, Kiyoshi Ohkawa, Koichi Shiraishi, Katsuhiko Aoki |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Survival Micelle Antibodies Mice 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Animals Humans Cytotoxic T cell Doxorubicin Cytotoxicity Micelles Antibiotics Antineoplastic Chemistry Carcinoma General Medicine Glutathione 030104 developmental biology Oncology Epidermoid carcinoma 030220 oncology & carcinogenesis Basigin Drug delivery Cancer research Conjugate medicine.drug |
| Zdroj: | Anticancer Research. 38 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.12353 |
| Popis: | Background CD147 (basigin/emmprin) is expressed on the surface of carcinoma cells. Materials and methods For studying the efficacy of CD147-targeting medicine on CD147-expressing cells, we studied the effect of anti-CD147-labeled polymeric micelles (CD147ab micelles) that encapsulated a conjugate of doxorubicin with glutathione (GSH-DXR), with specific accumulation and cytotoxicity against CD147-expressing A431 human epidermoid carcinoma cells, Ishikawa human endometrial adenocarcinoma cells, and PC3 human prostate carcinoma cells. Results By treatment of each cell type with CD147ab micelles for 1 h, a specific accumulation of CD147ab micelles in CD147-expressing cells was observed. In addition, the cytotoxicity of GSH-DXR-encapsulated micelles against each cell type was measured by treatment of the micelles for 1 h. The cytotoxic effect of CD147ab micelles carrying GSH-DXR was 3- to 10-fold higher for these cells than that of micelles without GSH-DXR. Conclusion These results suggest that GSH-DXR-encapsulated CD147ab micelles could serve as an effective drug delivery system to CD147-expressing carcinoma cells. |
| Databáze: | OpenAIRE |
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