Long noncoding RNA ATB promotes the epithelial−mesenchymal transition by upregulating the miR-200c/Twist1 axe and predicts poor prognosis in breast cancer

Autor: Xiao-Long Wei, Rong-Hui Li, Cui-Ping Guo, Yao-Chen Li, Jing Liu, Chang-Chun Shao, Min Chen, Wen-He Huang, Guo-Jun Zhang
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Cancer Research
Cohort Studies
Mice
0302 clinical medicine
Cell Movement
Transforming Growth Factor beta
RNA
Small Interfering
lcsh:Cytology
Nuclear Proteins
Cell migration
Middle Aged
Prognosis
Long non-coding RNA
Tumor Burden
Gene Expression Regulation
Neoplastic
030220 oncology & carcinogenesis
Lymphatic Metastasis
MCF-7 Cells
Biomarker (medicine)
Female
RNA
Long Noncoding
Signal Transduction
Adult
Epithelial-Mesenchymal Transition
Immunology
Mice
Nude
Breast Neoplasms
Article
03 medical and health sciences
Cellular and Molecular Neuroscience
Breast cancer
Downregulation and upregulation
medicine
Animals
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
lcsh:QH573-671
Survival analysis
Cell Proliferation
Neoplasm Staging
business.industry
Twist-Related Protein 1
Cell Biology
medicine.disease
Survival Analysis
Xenograft Model Antitumor Assays
MicroRNAs
030104 developmental biology
Cancer cell
Cancer research
business
Zdroj: Cell Death & Disease
Cell Death and Disease, Vol 9, Iss 12, Pp 1-16 (2018)
ISSN: 2041-4889
Popis: Recent studies indicate that the long noncoding RNA ATB (lncATB) can induce the epithelial−mesenchymal transition (EMT) in cancer cells, but the specific cellular targets of lncATB require further investigation. In the present study, the upregulation of lncATB in breast cancer cells was validated in a TGF-β-induced EMT model. Gain- and loss-of-function studies demonstrated that lncATB enhanced cell migration, invasion and clonogenicity in vitro and in vivo. LncATB promoted the EMT by acting as a sponge for the miR-200 family and restoring Twist1 expression. Subsequently, the clinical significance of lncATB was investigated in a cohort of breast cancer patients (N = 131). Higher lncATB expression was correlated with increased nodal metastasis (P = 0.036) and advanced clinical stage (P = 0.011) as well as shorter disease-free survival (P = 0.043) and overall survival (P = 0.046). These findings define Twist1 as a major target of lncATB in the induction of the EMT and highlight lncATB as a biomarker in breast cancer patients.
Databáze: OpenAIRE
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