| Popis: |
HDAC expression and cellular response to HDAC inhibition. A) qPCR of mRNA expression of HDAC 1-11 genes in normal human urothelial cells (NHU) and RT112, CAL29 and T24 bladder cancer cells. Relative gene expression was normalised to GAPDH levels (n=3); B) Effects of panobinostat on expression of selected HDAC proteins (n=2); C) Clonogenic assay representing cytotoxicity of mocetinostat in RT112, CAL29 and T24 bladder cancer cells, after 24 h incubation (n=3); D) Clonogenic assay representing cytotoxicity of TMP195 in RT112 cells, after 24 h incubation (n=3); E) Effect of panobinostat on acetylation levels of histone H3 lysine 18 (H3K18) at 0.5, 1, 2, 3 and 24 h incubation in RT112 cells (n=2); F) Quantification of western blots in Figure 6 (n=3 for MRE11, HDAC1 and NBS1, n=2 for HDAC2 and RAD51); G) HR assay results for TMP195 (n=3). |
