An understanding of potential and limitations of alginate/PLL microcapsules as a cell retention system for perfusion cultures
| Autor: | Harriet E. Cole, Ian W. Marison, Aurélie Demont |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Materials science Alginates Cell Cell Culture Techniques Pharmaceutical Science Capsules Bioengineering Nanotechnology CHO Cells 02 engineering and technology 01 natural sciences Cricetulus Colloid and Surface Chemistry Perfusion Culture Glucuronic Acid Cricetinae 010608 biotechnology Bioreactor Cell separation medicine Animals Polylysine Physical and Theoretical Chemistry chemistry.chemical_classification Hexuronic Acids Organic Chemistry High cell Polymer Cells Immobilized Mechanical resistance 021001 nanoscience & nanotechnology medicine.anatomical_structure chemistry Chemical engineering 0210 nano-technology Perfusion |
| Zdroj: | Journal of Microencapsulation. 33:80-88 |
| ISSN: | 1464-5246 0265-2048 |
| DOI: | 10.3109/02652048.2015.1134686 |
| Popis: | Microcapsules for high cell density culture of mammalian cells have found an increasing interest, however, the poor stability of the microcapsules and the lack of characterisation methods led to few quantitative results. Alginate-poly-L-lysine (PLL) microcapsules have been studied in detail in order to form a basis for comparison of capsules made from different polymers. Since the microcapsules can be easily retained in the bioreactor without the need for a cell separation device, high cell densities were achieved with a maximum of 4 × 10(7) cell/ml(microcapsules), corresponding to a colonisation of 5% of the internal capsule volume. Measurement of microcapsule integrity and mechanical resistance showed that alginate-PLL microcapsules are not suitable for perfusion cultures since they are very sensitive to media composition, mainly the presence of non-gelling ions that have a higher affinity for alginate than PLL and Ca(2+), leading to the leakage of PLL and Ca(2+), and to microcapsule rupture. |
| Databáze: | OpenAIRE |
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