The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization
| Autor: | Tom D. Wolkow, Shawn C. Verde, Lee G Elliott, Erin E. Baschal, Kuan J Chen, Matt J. Herring |
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| Rok vydání: | 2006 |
| Předmět: |
Cytoplasm
Cell cycle checkpoint DNA damage Cell Cycle Proteins Phleomycins Spindle Apparatus Biology Microtubules Evolution Molecular Chromosome segregation chemistry.chemical_compound Transformation Genetic Drug Resistance Fungal Microtubule Chromosome Segregation Thiabendazole Schizosaccharomyces Morphogenesis CHEK1 Phosphorylation lcsh:QH573-671 DNA Fungal Gene Nucleic Acid Synthesis Inhibitors lcsh:Cytology Intracellular Signaling Peptides and Proteins Cell Polarity Epistasis Genetic Cell Biology G2-M DNA damage checkpoint Actins Cell biology Genes cdc chemistry Benzimidazoles Carbamates Schizosaccharomyces pombe Proteins Protein Processing Post-Translational DNA DNA Damage Research Article |
| Zdroj: | BMC Cell Biology, Vol 7, Iss 1, p 32 (2006) BMC Cell Biology |
| ISSN: | 1471-2121 |
| DOI: | 10.1186/1471-2121-7-32 |
| Popis: | BackgroundDNA structure checkpoints are conserved eukaryotic signal transduction pathways that help preserve genomic integrity. Upon detecting checkpoint signals such as stalled replication forks or double-stranded DNA breaks, these pathways coordinate appropriate stress responses. Members of the PI-3 kinase related kinase (PIKK) family are essential elements of DNA structure checkpoints. In fission yeast, the Rad3 PIKK and its regulatory subunit Rad26 coordinate the detection of checkpoint signals with pathway outputs.ResultsWe found that untreatedrad26Δcells were defective for two microtubule-dependent processes: chromosome segregation and morphogenesis. Interestingly, cytoplasmic accumulation of Rad26-GFP occurred following treatment with microtubule destabilizing drugs, but not during treatment with the genotoxic agent Phleomycin. Cytoplasmic accumulation of Rad26-GFP depended on Rad24, a 14-3-3 protein also required for DNA structure checkpoints and morphogenesis. Results of over expression and epistasis experiments confirm that Rad26 and Rad24 define a response to microtubule destabilizing conditions.ConclusionTwo DNA structure checkpoint proteins with roles in morphogenesis define a response to microtubule destabilizing conditions. |
| Databáze: | OpenAIRE |
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