Targeted editing of tomato carotenoid isomerase reveals the role of 5′ UTR region in gene expression regulation
| Autor: | V. B. Reddy Lachagari, Rehna Augustine, Boney Kuriakose, Aju Antony, George Thomas, Navajeet Chakravartty, M. Uma Maheswari, Smitha Sukumaran, K. Lakshmi Jayaraj, Sweety Abraham, Somasekar Seshagiri, Subhash Narayanan, Moni John, Nitu Thulasidharan |
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| Rok vydání: | 2021 |
| Předmět: |
Chlorophyll
cis-trans-Isomerases 0106 biological sciences 0301 basic medicine Untranslated region Five prime untranslated region Agrobacterium Plant Science Biology 01 natural sciences 03 medical and health sciences Lycopene Solanum lycopersicum Gene Expression Regulation Plant CRISPR Gene Plant Proteins Gene Editing Genetics Regulation of gene expression Cas9 fungi Isomerase Gene food and beverages General Medicine Plants Genetically Modified Carotenoids Plant Leaves 030104 developmental biology Regulatory sequence Mutation 5' Untranslated Regions Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Cell Reports. 40:621-635 |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | A deletion created by CRISPR/Cas9 system in the 5' UTR of the carotenoid isomerase gene in tomato leads to downregulation of the gene resulting in the low conversion of prolycopene to lycopene. CRISPR/Cas9 based genome editing is an effective and useful tool adopted from the bacterial immune response system for altering specific, pre-determined DNA sequences in eukaryotes. Such targeted changes are finding wide application in human health as well as in precision breeding of crop plants for improved traits. Mutations in the coding and regulatory regions can have varying impacts on the function of the gene. In the current study, we demonstrate this on tomato carotenoid isomerase, a key gene in the carotenoid biosynthesis pathway. Mutations were generated in the 5' UTR and exon 1 of the carotenoid isomerase gene using CRISPR/Cas9 expression via Agrobacterium-mediated transformation of tomato variety Periyakulam 1 (PKM1). Molecular and biochemical studies demonstrate that CRISPR-mediated point mutations in the exon sequence lead to complete knockout of protein function whereas deletion in 5' UTR region lowers the expression of the gene leading to changes in plant phenotype. |
| Databáze: | OpenAIRE |
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