An environmentally regulated pilus-like appendage involved in Campylobacter pathogenesis
| Autor: | Patricia Guerry, Trevor J. Trust, Donald H. Burr, Ruijin Yao, Doig Peter C |
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| Rok vydání: | 1996 |
| Předmět: |
Mutant
Molecular Sequence Data medicine.disease_cause Microbiology Campylobacter jejuni Pilus Bile Acids and Salts Open Reading Frames Bacterial Proteins medicine Animals Amino Acid Sequence Cloning Molecular Molecular Biology Escherichia coli Heat-Shock Proteins biology Campylobacter Escherichia coli Proteins Serine Endopeptidases Ferrets Chromosomes Bacterial biology.organism_classification Molecular Weight Disease Models Animal Microscopy Electron Campylobacter coli Fimbriae Bacterial Female Campylobacter fetus Fimbriae Proteins DNA Probes Sequence Alignment Bacteria Bacterial Outer Membrane Proteins Peptide Hydrolases |
| Zdroj: | Molecular microbiology. 20(4) |
| ISSN: | 0950-382X |
| Popis: | Examination of strains of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. Various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. Morphologically, the pili were between 4 and 7 nm in width and were greater than 1 micron in length. A gene, termed pspA, which encodes a predicted protein resembling protease IV of Escherichia coli, was identified in C. jejuni strain 81-176. A site-specific insertional mutation within this gene resulted in the loss of pilus synthesis as determined by electron microscopy. Insertions upstream and downstream of the gene had no effect on pilus production. The non-piliated mutant of strain 81-176 showed no reduction in adherence to or invasion of INT 407 cells in vitro. However, this mutant, while still possessing the ability to colonize ferrets, caused significantly reduced disease symptoms in this animal model. |
| Databáze: | OpenAIRE |
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