Molecular Characterization Of Cytidine Monophospho-N-Acetylneuraminic Acid Hydroxylase (Cmah) Gene And Frequency Of Blood Types In Stray Cats Of Izmir, Turkey
| Autor: | Berna Bostanbaş, Cemal Ün, Ahmet Efe Köseoğlu, Sedef Erkunt Alak, Hüseyin Can, Mert Döşkaya, Umut Şahar, Serdar Baydarlı |
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| Rok vydání: | 2021 |
| Předmět: |
Turkey
CMAH gene Single-nucleotide polymorphism Cytidine Biology Neonatal isoerythrolysis QH426-470 Mixed Function Oxygenases 03 medical and health sciences chemistry.chemical_compound Exon 0302 clinical medicine Genetics SNP Animals Gene 030304 developmental biology 0303 health sciences CATS Stray cats Molecular biology Blood typing chemistry 030220 oncology & carcinogenesis Blood Group Antigens Cats TP248.13-248.65 Research Article Biotechnology |
| Zdroj: | BMC Genomics, Vol 22, Iss 1, Pp 1-7 (2021) BMC Genomics |
| Popis: | Background: Cytidine monophospho-n-acetylneuraminic acid hydroxylase (CMAH) gene associated with blood groups in cats encodes CMAH enzyme that converts Neu5Ac to Neu5Gc. Although variations in CMAH gene of pedigree cats have been revealed, the presence/lack of them in non-pedigree stray cats is unknown. Therefore, the present study aimed to investigate the variations in CMAH gene and the quantity of Neu5Ac and Neu5Gc on erythrocytes of non-pedigree stray cats (n:12) living in İzmir, Turkey. Also, the frequency of blood types was determined in 76 stray cats including 12 cats that were used for CMAH and Neu5A/Neu5Gc analysis. Results: In total, 14 SNPs were detected in 5’UTR as well as in exon 2, 4, 9, 10, 11 and 12 of CMAH gene. Among these SNPs, -495 C > T in 5’UTR was detected for the first time as heterozygous in type A and AB cats, and homozygous and heterozygous in type B cats. The remaining 13 that have been detected in previous studies were also found as homozygous or heterozygous. Both Neu5Gc and Neu5Ac were detected in type A and AB cats. In type B cats, only Neu5Ac was detected. Among two type AB cats, the level of Neu5Ac was found higher in cat carrying heterozygous form (T/C) of 1392T > C. The prevalence of type B cats (67.1 %) was higher than others. Conclusions: The presence of a new SNP as well as previous SNPs indicates that more variations can be found in stray cats with a more comprehensive study in the future. Also, the high prevalence of type B cats demonstrates the possible risk of neonatal isoerythrolysis among stray cats living in İzmir, Turkey. © 2021, The Author(s). 218Z105 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK This study was supported by a project given by The Scientific and Technological Research Council of Turkey (TÜBİTAK) (Project number: 218Z105) to C.Ü. The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. |
| Databáze: | OpenAIRE |
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