DNA damage by 8-methoxypsoralen plus near ultraviolet light (PUVA) and its repair in Escherichia coli: genetic analysis
| Autor: | I.B. Holland, S.I. Ahmad, J. Holland |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial DNA Repair Operon Ultraviolet Rays Mutant Microbial Sensitivity Tests Biology Toxicology medicine.disease_cause Bacterial Proteins Genetics medicine Escherichia coli Molecular Biology Gene Regulator gene Mutation Structural gene Mutagenesis Genetic Complementation Test Chromosome Mapping Molecular biology Molecular Weight Genes Bacterial Methoxsalen DNA Damage |
| Zdroj: | Mutation research. 254(3) |
| ISSN: | 0027-5107 |
| Popis: | Mutants of Escherichia coli, hyper-resistant and sensitive to 8-methoxypsoralen plus near ultraviolet light (PUVA) have been isolated and studied. Results show that a mutation, located at 57.2 min on the linkage map of E. coli, is responsible for the hyper-resistant phenotype. It is also responsible for the synthesis of a 55-kdal protein in high concentrations. In a wild-type cell the synthesis of this enzyme is inducible by mitomycin C. There are indications that the mutation may have occurred in a regulatory gene, puvR, and as a result the operon, including a putative puvA gene (the structural gene for the synthesis of the 55-kdal protein), is expressed constitutively. A model for the control of the PUV operon is proposed. |
| Databáze: | OpenAIRE |
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