Posttranscriptional Self-Regulation by the Lyme Disease Bacterium's BpuR DNA/RNA-Binding Protein
| Autor: | Alicia M. Chenail, Ashutosh Verma, Alyssa D. Antonicello, Grant S. Jones, Brian Stevenson, Nicholas A. Brown, Brandon L. Jutras |
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| Rok vydání: | 2013 |
| Předmět: |
Regulation of gene expression
Lyme Disease Messenger RNA Transcription Genetic biology Binding protein RNA-Binding Proteins Translation (biology) Gene Expression Regulation Bacterial Articles biology.organism_classification Microbiology Molecular biology Cell biology RNA Bacterial Prokaryotic DNA replication Borrelia burgdorferi Proteome Nucleic acid RNA Messenger RNA Processing Post-Transcriptional Molecular Biology Protein Binding |
| Zdroj: | Journal of Bacteriology. 195:4915-4923 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Bacteria require explicit control over their proteomes in order to compete and survive in dynamic environments. The Lyme disease spirocheteBorrelia burgdorferiundergoes substantial protein profile changes during its cycling between vector ticks and vertebrate hosts. In an effort to understand regulation of these transitions, we recently isolated and functionally characterized the borrelial nucleic acid-binding protein BpuR, a PUR domain-containing protein. We now report that this regulatory protein governs its own synthesis through direct interactions withbpuRmRNA.In vitroandin vivotechniques indicate that BpuR binds with high affinity and specificity to the 5′ region of its message, thereby inhibiting translation. This negative feedback could permit the bacteria to fine-tune cellular BpuR concentrations. These data add to the understanding of this newly described class of prokaryotic DNA- and RNA-binding regulatory proteins. |
| Databáze: | OpenAIRE |
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