Inhibited CD47 gene affects the clearance of acute myelogenous leukemia stem cells
| Autor: | Leiying Yang, Guangle Song |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Myeloid Apoptosis CD47 Antigen Biochemistry 03 medical and health sciences Myelogenous 0302 clinical medicine Biomarkers Tumor Tumor Cells Cultured medicine Humans CD90 RNA Small Interfering Molecular Biology Cell Proliferation Chemistry CD47 Myeloid leukemia Cell Biology Transfection medicine.disease Gene Expression Regulation Neoplastic Leukemia Myeloid Acute Leukemia 030104 developmental biology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Neoplastic Stem Cells Cancer research Myeloid Cell Leukemia Sequence 1 Protein Stem cell |
| Zdroj: | Journal of Cellular Biochemistry. 120:10303-10309 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | Objective To investigate the effect of targeted inhibition of CD47 gene expression on stem cell clearance in acute myeloid leukemia. Methods After the lentiviral CD47-siRNA was transfected into acute myelogenous leukemia stem cells (LSCs), the proliferative status of acute myelogenous LSCs was detected by cell counting kit-8, and the apoptosis of stem cells of acute myeloid leukemia was detected by annexin/propidium iodide flow assays. The expression of Bcl-2, Bcl-xl, MCL-1, PIK3p110β, and interleukin (IL)-3 in acute myeloid LSCs was detected by Western blot analysis and the activity of protein phosphatase 2A (PP2A) and the protein content of CD96 and CD90 were measured by enzyme-linked immunosorbent assay kits. Results After transfection of the lentivirus CD47-siRNA into acute myeloid LSCs, compared with the empty vector transfection group (control group), the cell viability of the CD47-siRNA transfection group was decreased, and the apoptosis rate was increased. Furthermore, the antiapoptotic protein Bcl-2, Bcl-xl, and MCL-1 and the content of IL-3 protein, CD96, and CD90 was decreased, whereas the activity of PIK3p110β and PP2A protein was increased. Conclusion Targeted inhibition of CD47 could inhibit the proliferation of myeloid LSCs, promote apoptosis, mobilize the cells into the cell cycle, and reduce the high expression of immune proteins on the cell surface, therefore providing a theoretical basis for the elimination and eradication of LSCs. |
| Databáze: | OpenAIRE |
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