Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1
| Autor: | Haruhisa Kawasaki, Ruth T. Yu, Taiga Suzuki, Kazuhiko Umesono, Hitoshi Ueda |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Amino Acid Motifs
Molecular Sequence Data Fushi Tarazu Transcription Factors Receptors Cytoplasmic and Nuclear Biology Steroidogenic Factor 1 Coactivator Animals Drosophila Proteins Amino Acid Sequence Transcription factor Cells Cultured Homeodomain Proteins Regulation of gene expression Genetics Orphan receptor Binding Sites Multidisciplinary fungi Biological Sciences Cell biology DNA-Binding Proteins Segmentation gene Nuclear receptor Insect Proteins Drosophila Drosophila Protein Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 98:12403-12408 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.221552998 |
| Popis: | Orphan receptors for whom cognate ligands have not yet been identified form a large subclass within the nuclear receptor superfamily. To address one aspect of how they might regulate transcription, we analyzed the mode of interaction between the Drosophila orphan receptor FTZ-F1 (NR5A3) and a segmentation gene product Fushi tarazu (FTZ). Strong interaction between these two factors was detected by use of the mammalian one- and two-hybrid interaction assays without addition of ligand. This interaction required the AF-2 core and putative ligand-binding domain of FTZ-F1 and the LXXLL motif of FTZ. The requirement of these elements was further confirmed by examination of their target gene expression in Drosophila embryos and observation of a cuticle phenotype in transgenic fly lines that express mutated factors. In Drosophila cultured cells, FTZ is required for FTZ-F1 activation of a FTZ-F1 reporter gene. These results reveal a resemblance in the mode of interaction between FTZ-F1 and FTZ and that of nuclear receptor-coactivator and indicate that direct interaction is required for regulation of gene expression by FTZ-F1. Thus, we propose that FTZ may represent a category of LXXLL motif-dependent coactivators for nuclear receptors. |
| Databáze: | OpenAIRE |
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