Modification of collagen IV by glucose or methylglyoxal alters distinct mesangial cell functions
| Autor: | Corina M. Borza, Ambra Pozzi, Billy G. Hudson, Nada Bulus, Peale Chuang, Dong Chen, Sergei V. Chetyrkin, Roy Zent, Paul A. Voziyan |
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| Rok vydání: | 2009 |
| Předmět: |
Collagen Type IV
Mesangial cell Arginine Cell growth Chemistry Methylglyoxal General Medicine Pyruvaldehyde Brief Communication Cell biology Collagen receptor Extracellular matrix chemistry.chemical_compound Mice Glucose Biochemistry Nephrology Glycation Cell Movement Mesangial Cells Animals Diabetic Nephropathies Cell adhesion Cells Cultured Cell Proliferation |
| Zdroj: | Journal of the American Society of Nephrology : JASN. 20(10) |
| ISSN: | 1533-3450 |
| Popis: | Diabetic nephropathy (DN) affects both glomerular cells and the extracellular matrix (ECM), yet the pathogenic mechanisms involving cell-matrix interactions are poorly understood. Glycation alters integrin-dependent cell-ECM interactions, and perturbation of these interactions results in severe renal pathology in diabetic animals. Here, we investigated how chemical modifications of the ECM by hyperglycemia and carbonyl stress, two major features of the diabetic milieu, affect mesangial cell functions. Incubation of collagen IV with pathophysiological levels of either the carbonyl compound methylglyoxal (MGO) or glucose resulted in modification of arginine or lysine residues, respectively. Mouse mesangial cells plated on MGO-modified collagen IV showed decreased adhesion and migration. Cells plated on glucose-modified collagen IV showed reduced proliferation and migration and increased collagen IV production. Inhibiting glucose-mediated oxidative modification of collagen IV lysine residues rescued the alterations in cell growth, migration, and collagen synthesis. We propose that diabetic ECM affects mesangial cell functions via two distinct mechanisms: modification of arginine residues by MGO inhibits cell adhesion, whereas oxidative modification of lysine residues by glucose inhibits cell proliferation and increases collagen IV production. These mechanisms may contribute to mesangial cell hypertrophy and matrix expansion in DN. |
| Databáze: | OpenAIRE |
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