Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants
| Autor: | Kamil Kuca, Farah K. Ahmed, Eugenie Nepovimova, Pooja Manchanda, Kamel A. Abd-Elsalam, Maninder Kaur, Anu Kalia |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Crops
Agricultural Agroinfiltration Computer science Agrobacterium QH301-705.5 transgene-free Computational biology Review Genome Catalysis Inorganic Chemistry Transformation Genetic Genome editing Gene Expression Regulation Plant CRISPR genome editing Physical and Theoretical Chemistry Biology (General) Molecular Biology Gene CRISPR/Cas9 QD1-999 Spectroscopy Plant Proteins Gene Editing transfer-DNA biology Cas9 Organic Chemistry targeted site modification General Medicine Plants biology.organism_classification Computer Science Applications Transformation (genetics) Chemistry Mutagenesis CRISPR-Cas Systems Genome Plant |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 10882, p 10882 (2021) International Journal of Molecular Sciences |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on Agrobacterium-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future. |
| Databáze: | OpenAIRE |
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