Indirect Suppression of Pulsatile LH Secretion by CRH Neurons in the Female Mouse
| Autor: | Xinhuai Liu, Allan E. Herbison, Robert Porteous, Isaiah Cheong, Siew Hoong Yip, Sabine Hessler |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Corticotropin-Releasing Hormone Down-Regulation Mice Transgenic Optogenetics Inhibitory postsynaptic potential Synaptic Transmission Gonadotropin-Releasing Hormone Mice Endocrinology Slice preparation Kisspeptin Arcuate nucleus Internal medicine medicine Animals Neurons GnRH Neuron Secretory Pathway Chemistry Arcuate Nucleus of Hypothalamus Luteinizing Hormone Mice Inbred C57BL medicine.anatomical_structure nervous system Pulsatile Flow Female Neuron Luteinizing hormone hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 162 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Acute stress is a potent suppressor of pulsatile luteinizing hormone (LH) secretion, but the mechanisms through which corticotrophin-releasing hormone (CRH) neurons inhibit gonadotropin-releasing hormone (GnRH) release remain unclear. The activation of paraventricular nucleus (PVN) CRH neurons with Cre-dependent hM3Dq in Crh-Cre female mice resulted in the robust suppression of pulsatile LH secretion. Channelrhodopsin (ChR2)-assisted circuit mapping revealed that PVN CRH neuron projections existed around kisspeptin neurons in the arcuate nucleus (ARN) although many more fibers made close appositions with GnRH neuron distal dendrons in the ventral ARN. Acutely prepared brain slice electrophysiology experiments in GnRH- green fluorescent protein (GFP) mice showed a dose-dependent (30 and 300 nM CRH) activation of firing in ~20% of GnRH neurons in both intact diestrus and ovariectomized mice with inhibitory effects being uncommon ( |
| Databáze: | OpenAIRE |
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