Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia
| Autor: | R Allsop, A Purewal, N. Edington, P M Chesters |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Immunology
In situ hybridization Biology Polymerase Chain Reaction Microbiology law.invention Trigeminal ganglion law Virology Virus latency Leukocytes medicine Animals Horses RNA Messenger Gene In Situ Hybridization Polymerase chain reaction Southern blot medicine.disease Molecular biology Virus Latency Blotting Southern Trigeminal Ganglion Insect Science Lymph CD8 Research Article Herpesvirus 1 Equid |
| Zdroj: | Journal of Virology. 71:3437-3443 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.71.5.3437-3443.1997 |
| Popis: | Results from Southern hybridization and PCR amplification experiments using a randomly synthesized reverse transcription-PCR product showed that peripheral blood leukocytes from horses showing no clinical signs of disease expressed a putative latency-associated transcript antisense to and overlapping the 3' end of the equid herpesvirus 1 (EHV-1) immediate-early gene (gene 64). A PCR product derived from this transcript has > or =96% identity with the published EHV-1 sequence. In situ hybridization studies of equine bronchial lymph nodes corroborated these findings and are consistent with reactivation data (D. A. Smith, A. Hamblin, and N. Edington, unpublished data), indicating that EHV-1 latency is established predominantly in CD5+/CD8+ leukocytes. |
| Databáze: | OpenAIRE |
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