Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense
Autor: | Shengming Sun, H T Fu, Ya-Ping Zhang, Yiwei Xiong, Shubo Jin, Xianping Ge, Sufei Jiang, Yongsheng Gong, Wenyi Zhang, Hui Qiao |
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Rok vydání: | 2015 |
Předmět: |
Male
DNA Complementary Molecular Sequence Data Biology Homology (biology) Arthropod Proteins Sex Factors Rivers Rapid amplification of cDNA ends Testis Genetics Animals Amino Acid Sequence Cloning Molecular Molecular Biology Gene Phylogeny Ganglion Cysts Regulation of gene expression Dosage compensation Base Sequence Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Myocardium Ovary Metamorphosis Biological Gene Expression Regulation Developmental Sequence Analysis DNA General Medicine Molecular biology Gene expression profiling Larva Prawn Female Palaemonidae Macrobrachium nipponense |
Zdroj: | Genetics and Molecular Research. 14:3106-3120 |
ISSN: | 1676-5680 |
Popis: | In this study, male-specific lethal 3 homolog (Mnmsl3) was cloned and characterized from the freshwater prawn Macrobrachium nipponense (Crustacea: Decapoda: Palaemonidae) by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnmsl3 showed high-sequence homology to the insect Msl3 and contained a conserved chromatin organization modifier domain and an MORF4-related gene domain. Real-time quantitative reverse transcription-polymerase chain reaction showed that the Mnmsl3 gene was expressed in all the investigated tissues, with the highest level of expression in the testis. The expression level of Mnmsl3 between males and females was different in the gonad (testis or ovary), abdominal ganglion, and heart. The results revealed that the Mnmsl3 gene might play roles in regulating chromatin and in dosage compensation of M. nipponense. Real-time quantitative reverse transcription-polymerase chain reaction also revealed that Mnmsl3 mRNA expression was significantly increased in both 5 and 20 days post-larvae after metamorphosis, suggesting that Mnmsl3 plays complex and important roles in the early embryonic development and sex differentiation of M. nipponense. |
Databáze: | OpenAIRE |
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