Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies
| Autor: | Zaheed Husain, Karuppiah Thilakavathy, Norshariza Nordin, Mansour Zamanpoor, Rozita Rosli, Mohd Nazri Yazid |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Gestational hypertension medicine.medical_specialty Anemia Gestational Age Real-Time Polymerase Chain Reaction Fetus Pregnancy Diabetes mellitus Internal medicine medicine Humans Anemia Iron-Deficiency business.industry Obstetrics and Gynecology Gestational age DNA Hypertension Pregnancy-Induced medicine.disease Sex-Determining Region Y Protein Pregnancy Complications Gestational diabetes Diabetes Gestational Endocrinology Iron-deficiency anemia Pediatrics Perinatology and Child Health Female business Blood Chemical Analysis |
| Zdroj: | The Journal of Maternal-Fetal & Neonatal Medicine. 26:960-966 |
| ISSN: | 1476-4954 1476-7058 |
| DOI: | 10.3109/14767058.2013.766710 |
| Popis: | To quantify circulating fetal DNA (fDNA) levels in the second and third trimesters of normal healthy pregnant individuals and pregnant women with the following clinical conditions: gestational diabetes mellitus (GDM), iron deficiency anemia and gestational hypertension (GHT).The SRY gene located on the Y chromosome was used as a unique fetal marker. The fDNA was extracted from maternal plasma and the SRY gene concentrations were measured by quantitative real-time polymerase chain reaction (PCR) amplification using TaqMan dual labeled probe system.No significant differences were observed in the mean fDNA concentration between normal and GDM pregnancy samples (p0.05) and also between normal and anemic pregnancy samples (p0.05) in both trimesters, but significant differences were observed between the third trimester normal and GHT pregnancy samples (p = 0.001). GDM and iron deficiency anemia do not affect the levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma.Increased amount of circulating fDNA in maternal plasma could be used for early identification of adverse pregnancies. GDM and anemia do not affect the levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma. Hence, the elevated fDNA values could be used as a potential screening marker in pregnancies complicated with GHT but not with GDM and iron deficiency anemia. |
| Databáze: | OpenAIRE |
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