Myocardial Homing of Nonmobilized Peripheral-Blood CD34+ Cells After Intracoronary Injection
| Autor: | Micheline Lambermont, Guy Berkenboom, Serge Goldman, Michel Toungouz, Philippe Unger, Dominique Egrise, Nicolas Preumont, Didier Blocklet, Eric Stoupel, Michel Goldman, Jean-Paul Degaute |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Myocardial Infarction CD34 Infarction Antigens CD34 Biology Coronary Restenosis Restenosis Cell Movement Internal medicine medicine Humans Myocardial infarction Radionuclide Imaging Aged Myocardium Hematopoietic Stem Cell Transplantation Hematopoietic stem cell Cell Biology Middle Aged Hematopoietic Stem Cells medicine.disease Coronary Vessels Hematopoietic Stem Cell Mobilization medicine.anatomical_structure Positron-Emission Tomography Immunology Cardiology Feasibility Studies Molecular Medicine Female Stem cell Cytapheresis Follow-Up Studies Developmental Biology Homing (hematopoietic) |
| Zdroj: | Stem Cells. 24:333-336 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1634/stemcells.2005-0201 |
| Popis: | Granulocyte– colony-stimulating factor administered for autologous hematopoietic stem cell isolation from blood may favor restenosis in patients implanted after acute myocardial infarction (AMI). We therefore tested the isolation of peripheral-blood CD34+ cells without mobilization in six patients with AMI. After large-volume cytapheresis and positive CD34+ cell selection, 3.6 to 27.6 million CD34+ cells were obtained. We performed intra-coronary implantation of these cells and recorded no restenosis or arrhythmia. We used positron emission tomography (PET) to assess myocardial-labeled CD34+ cell homing, which accounted for 5.5% of injected cells 1 hour after implantation. In conclusion, large amounts of CD34+ cells, in the range reported in previous studies, can be obtained from nonmobilized peripheral blood. PET with [18F]-fluorodeoxyglucose cell labeling is an efficient imaging method for homing assessment. |
| Databáze: | OpenAIRE |
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