Co-immunisation with a plasmid DNA cocktail primes mice against anthrax and plague
Autor: | R.J Beedham, Stuart D. Perkins, Alice M. Bennett, Les Baillie, Ethel Diane Williamson, Julie Miller |
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Rok vydání: | 2002 |
Předmět: |
Yersinia pestis
T-Lymphocytes Immunization Secondary Priming (immunology) Anthrax Vaccines In Vitro Techniques Biology law.invention DNA vaccination Anthrax Mice chemistry.chemical_compound Plasmid Bacterial Proteins Antigen law Vaccines DNA Animals Vaccines Combined Antigens Bacterial Mice Inbred BALB C Plague Plague Vaccine General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health biology.organism_classification Antibodies Bacterial Virology Recombinant Proteins Bacillus anthracis Infectious Diseases chemistry Immunoglobulin G Recombinant DNA Molecular Medicine Female DNA |
Zdroj: | Vaccine. 20:2933-2941 |
ISSN: | 0264-410X |
DOI: | 10.1016/s0264-410x(02)00232-3 |
Popis: | The protective antigen (PA) of Bacillus anthracis and the V antigen of Yersinia pestis are potent immunogens and candidate vaccine sub-units. When plasmid DNA encoding either PA or V antigen was used to immunise the Balb/c mouse, a low serum IgG titre was detected (log (10)1.0 or less) which was slightly increased by boosting with plasmid DNA. However, when mice immunised with plasmid DNA were later boosted with the respective recombinant protein, a significant increase in titre (up to 100-fold) was observed. Mice primed with a combination of each plasmid and boosted with a combination of the recombinant proteins, were fully protected (6/6) against challenge with Y. pestis. This compared favourably with mice primed only with plasmid DNA encoding the V antigen and boosted with rV, which were partially protected (3/6) against homologous challenge or with mice primed and boosted with plasmid DNA encoding the V antigen which were poorly protected (1/6). Combined immunisation with the two plasmid DNA constructs followed by boosting with a combination of the encoded recombinant proteins enhanced the protective immune response to Y. pestis compared with priming only with plasmid DNA encoding the V antigen and boosting with rV. This enhancement may be due to the effect of CpG motifs known to be present in the plasmid DNA construct encoding PA. |
Databáze: | OpenAIRE |
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