An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells
| Autor: | Isabelle Momas, Sophie Achard, Charles Persoz, Nathalie Seta, Christopher Leleu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cell Survival
Inflammation Toxicology Models Biological Cell Line Formaldehyde medicine Interleukin 8 Respiratory system Lung Chemokine CCL2 Sensitization Inhalation exposure A549 cell Volatile Organic Compounds Interleukin-6 Tumor Necrosis Factor-alpha Chemistry Interleukin-8 Epithelial Cells Environmental Exposure General Medicine Environmental exposure medicine.anatomical_structure Immunology Tumor necrosis factor alpha Inflammation Mediators medicine.symptom |
| Zdroj: | Toxicology Letters. 195:99-105 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2010.03.003 |
| Popis: | Asthma is a public health problem worldwide, and indoor air pollution considered to be a potential etiology. New tools need to be developed to study the effects of air pollutants in vitro and modelize inhalation exposure. This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells, and apply this to gaseous formaldehyde (FA). A549 cells were exposed using the direct exposure device (air/liquid interface) to FA without, after and before TNFalpha (1 ng/mL) sensitization. 24h after exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed. No effects on cellular viability were observed for concentrations < or =50 microg/m(3). After TNFalpha sensitization, FA-exposure induced a significant increase in IL-8 (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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