The combination of conjugated linoleic acid (CLA) and extra virgin olive oil increases mitochondrial and body metabolism and prevents CLA-associated insulin resistance and liver hypertrophy in C57Bl/6 mice
| Autor: | Carlos Curti, Luciane C. Alberici, Bruno G. Teodoro, Flávia G. Baraldi, Sérgio Akira Uyemura, Ieda M.R. Prado, Fernanda Cardoso, Felipe M. Dalalio, Tatiane M. Vicentini, Carlos Roberto Porto Dechandt |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Antioxidant Endocrinology Diabetes and Metabolism Linoleic acid Conjugated linoleic acid medicine.medical_treatment Clinical Biochemistry Mitochondria Liver White adipose tissue Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound Insulin resistance Internal medicine medicine Animals Uncoupling protein Linoleic Acids Conjugated Olive Oil Molecular Biology Nutrition and Dietetics biology food and beverages Hypertrophy Metabolism medicine.disease Mice Inbred C57BL 030104 developmental biology Endocrinology Liver chemistry Catalase MITOCÔNDRIAS biology.protein lipids (amino acids peptides and proteins) Insulin Resistance Energy Metabolism |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0955-2863 |
| Popis: | Clinical conditions associated with obesity can be improved by daily intake of conjugated linoleic acid (CLA) or extra virgin olive oil (EVOO). Here we investigated whether dietary supplementation with CLA and EVOO, either alone or in combination, changes body metabolism associated with mitochondrial energetics. Male C57Bl/6 mice were divided into one of four groups: CLA (1:1 cis-9, trans-11:trans-10, cis-12; 18:2 isomers), EVOO, CLA plus EVOO or control (linoleic acid). Each mouse received 3 g/kg body weight of the stated oil by gavage on alternating days for 60 days. Dietary supplementation with CLA, alone or in combination with EVOO: (a) reduced the white adipose tissue gain; (b) increased body VO2 consumption, VCO2 production and energy expenditure; (c) elevated uncoupling protein (UCP)-2 expression and UCP activity in isolated liver mitochondria. This organelle, when energized with NAD(+)-linked substrates, produced high amounts of H2O2 without inducing oxidative damage. Dietary supplementation with EVOO alone did not change any metabolic parameter, but supplementation with CLA itself promoted insulin resistance and elevated weight, lipid content and acetyl-CoA carboxylase-1 expression in liver. Interestingly, the in vivo antioxidant therapy with N-acetylcysteine abolished the CLA-induced rise of body metabolism and liver UCP expression and activity, while the in vitro antioxidant treatment with catalase mitigated the CLA-dependent UCP-2 expression in hepatocytes; these findings suggest the participation of an oxidative-dependent pathway. Therefore, this study clarifies the mechanisms by which CLA induces liver UCP expression and activity, and demonstrates for the first time the beneficial effects of combined CLA and EVOO supplementation. |
| Databáze: | OpenAIRE |
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