Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Autor: Fabian Davamani, Teik Hwa Ong, Kah Hay Yuen, Stephen Ambu, Rajinikanth Paruvathanahalli Siddalingam, Srinivasan Ramamurthy, Ebenezer Chitra
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Gene Expression
lcsh:Medicine
02 engineering and technology
Pathology and Laboratory Medicine
Chitosan
chemistry.chemical_compound
Medicine and Health Sciences
Nanotechnology
lcsh:Science
Multidisciplinary
Pinocembrin
biology
Antimicrobials
Organic Compounds
Drugs
021001 nanoscience & nanotechnology
Antimicrobial
Bacterial Pathogens
Chemistry
Medical Microbiology
Physical Sciences
Engineering and Technology
Pathogens
0210 nano-technology
Research Article
Biotechnology
Enterococcus Faecalis
Microbiology
Enterococcus faecalis
03 medical and health sciences
Microbial Control
Genetics
Microbial Pathogens
Pharmacology
Bacteria
Ethanol
Organic Chemistry
lcsh:R
Biofilm
Organisms
Chemical Compounds
Biology and Life Sciences
Bacteriology
Propolis
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
030104 developmental biology
chemistry
Biofilms
Alcohols
Antibiotic Resistance
Bionanotechnology
Nanoparticles
Antibacterials
lcsh:Q
Antimicrobial Resistance
Kaempferol
Bacterial Biofilms
Enterococcus
Zdroj: PLoS ONE, Vol 12, Iss 3, p e0174888 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC) analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.
Databáze: OpenAIRE
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