The 5′-HS4 chicken β-globin insulator is a CTCF-dependent nuclear matrix-associated element
| Autor: | Timur M. Yusufzai, Gary Felsenfeld |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
CCCTC-Binding Factor
Erythrocytes Molecular Sequence Data In Vitro Techniques Biology Animals Nuclear Matrix Binding site Nuclear protein Nucleoplasmins Scaffold/matrix attachment region Locus control region Nucleophosmin Binding Sites Multidisciplinary Base Sequence Nuclear Proteins DNA Biological Sciences Locus Control Region Phosphoproteins Nuclear matrix Molecular biology Globins Cell biology DNA-Binding Proteins Repressor Proteins CTCF Phosphoprotein Chickens |
| Zdroj: | Proceedings of the National Academy of Sciences. 101:8620-8624 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0402938101 |
| Popis: | The protein CTCF plays an essential role in the action of a widely distributed class of vertebrate enhancer-blocking insulators, of which the first example was found in a DNA sequence element, HS4, at the 5′ end of the chicken β-globin locus. HS4 contains a binding site for CTCF that is necessary and sufficient for insulator action. Purification of CTCF has revealed that it interacts with proteins involved in subnuclear architecture, notably nucleophosmin, a 38-kDa nucleolar phosphoprotein that is concentrated in nuclear matrix preparations. In this report we show that both CTCF and the HS4 insulator element are incorporated in the matrix; HS4 incorporation depends on the presence of an intact CTCF-binding site. However the DNA sequence in the neighborhood of HS4 is not like that of canonical matrix attachment regions, and its incorporation into the matrix fraction is not sensitive to ribonuclease, suggesting that the insulator is a distinct matrix-associated element. |
| Databáze: | OpenAIRE |
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