Prospective evaluation of the OKN K-SeT assay, a new multiplex immunochromatographic test for the rapid detection of OXA-48-like, KPC and NDM carbapenemases
| Autor: | Te-Din Huang, Rémy A. Bonnin, Agnès B Jousset, Stéphanie Evrard, Pierre Bogaerts, Youri Glupczynski, Laurent Dortet, Thierry Naas |
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| Přispěvatelé: | UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Immunochromatographic test Microbial Sensitivity Tests Carbapenem-resistant enterobacteriaceae Sensitivity and Specificity Microbiology Rapid detection Chromatography Affinity beta-Lactamases Prospective evaluation 03 medical and health sciences Bacterial Proteins ENTEROBACTERIACEAE 1108 Medical Microbiology polycyclic compounds Humans Medicine Pharmacology (medical) Multiplex Prospective Studies Pharmacology & Pharmacy Original Research Pharmacology Bacteriological Techniques Science & Technology business.industry Enterobacteriaceae Infections Diagnostic test biochemical phenomena metabolism and nutrition Editor's Choice Clinical microbiology Carbapenem-Resistant Enterobacteriaceae Infectious Diseases CARBA NP TEST BACTERIA 1115 Pharmacology And Pharmaceutical Sciences business Life Sciences & Biomedicine 0605 Microbiology |
| Zdroj: | Journal of Antimicrobial Chemotherapy Journal of Antimicrobial Chemotherapy, Vol. 72, no. 7, p. 1955-1960 (2017) |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkx089 |
| Popis: | OBJECTIVES: There is an urgent need for accurate and fast diagnostic tests capable of identifying carbapenemase producers. Here, we assessed the performance of a new multiplex lateral flow assay (OKN K -SeT) for the rapid detection of OXA-48-like, KPC and NDM carbapenemase-producing Enterobacteriaceae from culture colonies. METHODS: Two hundred collection isolates with characterized β-lactamase content and 183 non-duplicate consecutive isolates referred to two National Reference Centres over a 2 month period in 2016 were used to evaluate the OKN K -SeT assay. RESULTS: The assay correctly detected all 42 OXA-48-like-, 27 KPC- and 30 NDM-producing isolates from the collection panel, including 7 isolates that co-produced NDM and OXA-181 carbapenemases. No cross-reactivity was observed with non-targeted carbapenemases ( n = 41) or with non-carbapenemase producers ( n = 60). Prospectively, all OXA-48-like ( n = 69), KPC ( n = 9) and NDM ( n = 19) carbapenemase-producing Enterobacteriaceae isolates were correctly detected, while 11 carbapenemase producers not targeted by the assay went undetected [VIM ( n = 8) and OXA-23/OXA-58-like ( n = 3)]. Overall, the sensitivity and specificity of the assay were 100%. CONCLUSIONS: The OKN assay is efficient, rapid and easy to implement in the workflow of a clinical microbiology laboratory for the confirmation of OXA-48, NDM and KPC carbapenemases. This test represents a powerful diagnostic tool as it enables the rapid detection of the most clinically important carbapenemases without the need for more costly and less frequently available molecular assays. |
| Databáze: | OpenAIRE |
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