Huperzine A production by Paecilomyces tenuis YS-13, an endophytic fungus isolated from Huperzia serrata
Autor: | Jingqian Su, Minhe Yang |
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Rok vydání: | 2015 |
DOI: | 10.6084/m9.figshare.1254110.v2 |
Popis: | Huperzine A (HupA), a naturally occurring alkaloid in the plant family Huperziaceae, has drawn great interest for its potential application in Alzheimer disease therapy. Our primary objective was to identify alkaloid- and HupA-producing fungi from the Chinese folk herb, Huperzia serrata. We established a rapid and efficient model for screening HupA-producing endophytic fungal strains. The presence of HupA in Paecilomyces tenuis YS-13 was analysed by thin-layer chromatography, high-performance liquid chromatography and mass spectrometry. The fermentation yield of HupA was 21.0 μg/L, and the IC50 of the crude extract of YS-13 fermentation broth was 1.27 ± 0.04 mg/mL. This is the first report of P. tenuis as a HupA-producing endophyte isolated from Huperziaceae. |
Databáze: | OpenAIRE |
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