Mangiferin ameliorates insulin resistance by inhibiting inflammation and regulatiing adipokine expression in adipocytes under hypoxic condition
| Autor: | Jing-Hua Xu, Fang Huang, Dan-Dan Yan, Chao-Qiang Yang, Kang Liu, Baolin Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Xanthones Glucose uptake Adipose tissue Adipokine Inflammation Biology Proinflammatory cytokine Mice 03 medical and health sciences chemistry.chemical_compound Insulin resistance Adipokines 3T3-L1 Cells Internal medicine Drug Discovery Adipocytes medicine Animals Humans Insulin Lipolysis Mangiferin Tumor Necrosis Factor-alpha NF-kappa B General Medicine Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Cell Hypoxia Oxygen Glucose 030104 developmental biology Endocrinology Complementary and alternative medicine chemistry Insulin Resistance medicine.symptom |
| Zdroj: | Chinese Journal of Natural Medicines. 15:664-673 |
| ISSN: | 1875-5364 |
| DOI: | 10.1016/s1875-5364(17)30095-x |
| Popis: | Adipose tissue hypoxia has been recognized as the initiation of insulin resistance syndromes. The aim of the present study was to investigate the effects of mangiferin on the insulin signaling pathway and explore whether mangiferin could ameliorate insulin resistance caused by hypoxia in adipose tissue. Differentiated 3T3-L1 adipocytes were incubated under normal and hypoxic conditions, respectively. Protein expressions were analyzed by Western blotting. Inflammatory cytokines and HIF-1-dependent genes were tested by ELISA and q-PCR, respectively. The glucose uptake was detected by fluorescence microscopy. HIF-1α was abundantly expressed during 8 h of hypoxic incubation. Inflammatory reaction was activated by up-regulated NF-κB phosphorylation and released cytokines like IL-6 and TNF-α. Glucose uptake was inhibited and insulin signaling pathway was damaged as well. Mangiferin substantially inhibited the expression of HIF-1α. Lactate acid and lipolysis, products released by glycometabolism and lipolysis, were also inhibited. The expression of inflammatory cytokines was significantly reduced and the damaged insulin signaling pathway was restored to proper functional level. The glucose uptake of hypoxic adipocytes was promoted and the dysfunction of adipocytes was relieved. These results showed that mangiferin could not only improve the damaged insulin signaling pathway in hypoxic adipocytes, but also ameliorate inflammatory reaction and insulin resistance caused by hypoxia. |
| Databáze: | OpenAIRE |
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