Calycosin Alleviates Sepsis-Induced Acute Lung Injury via the Inhibition of Mitochondrial ROS-Mediated Inflammasome Activation
| Autor: | Juan Yu, Yingyan Tang, Yuanbao Cao, Hongjuan Hu, Wenjia Ma, Rui Bao, Yao Sun, Xiuying Hong, Yu Xia, Kailun Qin |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mitochondrial ROS
Lipopolysaccharide Caspase 1 calycosin RM1-950 Lung injury Pharmacology medicine.disease_cause Superoxide dismutase sepsis chemistry.chemical_compound inflammasome medicine Pharmacology (medical) chemistry.chemical_classification reactive oxygen species Reactive oxygen species biology Inflammasome chemistry acute lung injury biology.protein Therapeutics. Pharmacology Oxidative stress medicine.drug |
| Zdroj: | Frontiers in Pharmacology, Vol 12 (2021) |
| ISSN: | 1663-9812 |
| DOI: | 10.3389/fphar.2021.690549/full |
| Popis: | Sepsis-induced acute lung injury (ALI) culminates in multiple organ failure via uncontrolled inflammatory responses and requires effective treatment. Herein, we aimed to investigate the effect of calycosin (CA), a natural isoflavonoid, on sepsis-induced ALI. CA attenuated lipopolysaccharide (LPS) and cecal ligation and puncture (CLP)-induced structural damage and inflammatory cell infiltration in lung tissues by histopathological analysis. CA significantly reduced lung wet/dry ratio, inflammatory cell infiltration in bronchoalveolar lavage fluid, and myeloperoxidase activity. Moreover, CA improved the survival of septic mice. CA also substantially inhibited interleukin (IL)-1β and IL-18 levels and cleaved caspase 1 expression and activity in lung tissues. Additionally, CA markedly suppressed oxidative stress by increasing levels of superoxide dismutase and glutathione while decreasing malondialdehyde. In vitro assay showed that CA significantly inhibited LPS-induced IL-1β and IL-18 levels and cleaved caspase 1 expression and activity in BMDMs. Moreover, CA blocked the interaction among NLRP3, ASC, and caspase 1 in LPS-treated cells. CA markedly reduced mitochondrial ROS levels. Significantly, compared with CA treatment, the combination of CA and MitoTEMPO (mitochondria-targeted antioxidant) did not further reduce the IL-1β and IL-18 levels and cleaved caspase 1 expression and activity and decreased mitochondrial ROS levels. Collectively, the inhibition of mitochondrial ROS-mediated NLRP3 inflammasome activation contributes to the protective effects of CA, which may be considered a potential therapeutic agent for septic ALI. |
| Databáze: | OpenAIRE |
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