MLVA and MLST typing of Brucella from Qinghai, China
| Autor: | Fang Zhao, Junying Ma, Yu-Min Qin, Hongyan Zhao, Xuefei Zhang, Hu Wang, Buyun Cui, Liqing Xu, Hai Jiang, Gonghua Lin, Gui-Ying Hu, Dongri Piao |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Veterinary medicine China Genotype 030106 microbiology Population Short Report Locus (genetics) Brucella Minisatellite Repeats Biology Multiple Loci VNTR Analysis Brucellosis 03 medical and health sciences medicine Animals Humans Zoonotic host Typing education Phylogeny education.field_of_study Sheep Public Health Environmental and Occupational Health General Medicine Ruminants biology.organism_classification medicine.disease bacterial infections and mycoses 030104 developmental biology Infectious Diseases Multilocus sequence typing Cattle Molecular identification Multilocus Sequence Typing |
| Zdroj: | Infectious Diseases of Poverty |
| ISSN: | 2049-9957 |
| DOI: | 10.1186/s40249-016-0123-z |
| Popis: | Background The Qinghai-Tibet Plateau (QTP) of China is an extensive pastoral and semi-pastoral area, and because of poverty and bad hygiene conditions, Brucella is highly prevalent in this region. In order to adequately prevent this disease in the QTP region it is important to determine the identity of Brucella species that caused the infection. Methods A total of 65 Brucella isolates were obtained from human, livestock and wild animals in Qinghai, a Chinese province in east of the QTP. Two molecular typing methods, MLVA (multi-locus variable-number tandem-repeat analysis) and MLST (multi locus sequence typing) were used to identify the species and genotypes of these isolates. Findings Both MLVA and MLST typing methods classified the 65 isolates into three species, B. melitensis, B. abortus and B. suis, which included 60, 4 and 1 isolates respectively. The MLVA method uniquely detected 34 (Bm01 ~ Bm34), 3 (Ba01 ~ Ba03), and 1 (Bs01) MLVA-16 genotypes for B. melitensis, B. abortus and B. suis, respectively. However, none of these genotypes exactly matched any of the genotypes in the Brucella2012 MLVA database. The MLST method identified five known ST types: ST7 and ST8 (B. melitensis), ST2 and ST5 (B. abortus), and ST14 (B. suis). We also detected a strain with a mutant type (3-2-3-2-?-5-3-8-2) of ST8 (3-2-3-2-1-5-3-8-2). Extensive genotype-sharing events could be observed among isolates from different host species. Conclusions There were at least three Brucella (B. melitensis, B. abortus and B. suis) species in Qinghai, of which B. melitensis was the predominant species in the area examined. The Brucella population in Qinghai was very different from other regions of the world, possibly owing to the unique geographical characteristics such as extremely high altitude in QTP. There were extensive genotype-sharing events between isolates obtained from humans and other animals. Yaks, sheep and blue sheep were important zoonotic reservoirs of brucellosis causing species found in humans. Electronic supplementary material The online version of this article (doi:10.1186/s40249-016-0123-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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