Amiloride-inhibited Na+-H+ exchange in human kidney medulla microsomes
| Autor: | E. F. LaBelle |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Nigericin
Physiology Sodium Ionophore chemistry.chemical_element Amiloride chemistry.chemical_compound Microsomes medicine Humans Kidney Medulla Reabsorption Vesicle Osmolar Concentration Acridine orange Proteins Biological Transport Hydrogen-Ion Concentration Membrane transport Acridine Orange Ion Exchange chemistry Biochemistry Biophysics Hydrogen medicine.drug |
| Zdroj: | American Journal of Physiology-Renal Physiology. 251:F232-F237 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.1986.251.2.f232 |
| Popis: | Membrane vesicles from the medulla of the human kidney are capable of amiloride-inhibited sodium-proton exchange. Sodium uptake into the vesicles is stimulated by low intravesicular pH (6.0) and inhibited by both low extravesicular pH (6.0) and amiloride (2 mM). Sodium uptake is a linear function of the vesicular protein concentration and can be reversed by raising the osmotic strength of the solution to shrink the vesicles or by adding the ionophore nigericin. Acridine orange was accumulated by the vesicles in response to a pH gradient (pH 6.0, inside, 8.0 outside), and the accumulation of acridine orange could be reduced by increased extravesicular sodium. Thus sodium could stimulate proton movement just as protons could stimulate sodium movement across the vesicular membrane. |
| Databáze: | OpenAIRE |
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