Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping

Autor:	Holly-May Lewis, Catia Costa, Véronique Dartois, Firat Kaya, Mark Chambers, Janella de Jesus, Vladimir Palitsin, Roger Webb, Melanie J. Bailey
Rok vydání:	2022
Předmět:	Lipidomics Animals Rabbits Lipids Biomarkers Mass Spectrometry Chromatography Liquid Analytical Chemistry
Zdroj:	Analytical Chemistry. 94:11798-11806
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.2c01940
Popis:	Elemental imaging is widely used for imaging cells and tissues but rarely in combination with organic mass spectrometry, which can be used to profile lipids and measure drug concentrations. Here, we demonstrate how elemental imaging and a new method for spatially resolved lipidomics (DAPNe-LC-MS, based on capillary microsampling and liquid chromatography mass spectrometry) can be used in combination to probe the relationship between metals, drugs, and lipids in discrete areas of tissues. This new method for spatial lipidomics, reported here for the first time, has been applied to rabbit lung tissues containing a lesion (caseous granuloma) caused by tuberculosis infection. We demonstrate how elemental imaging with spatially resolved lipidomics can be used to probe the association between ion accumulation and lipid profiles and verify local drug distribution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96f3f8c5375052c979caff65193a731e https://doi.org/10.1021/acs.analchem.2c01940 Zobrazit plný text záznamu