Tubeimoside-1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt-mediated signaling pathways

Autor:	Zhong‑Xia Chu, Ming‑Ming Wang, Yue Ma, Zhuang Shi, Hai‑Tang Xie, Li‑Juan Cao
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Cancer Research Cell Survival Cell Biochemistry 03 medical and health sciences Phosphatidylinositol 3-Kinases 0302 clinical medicine tubeimoside-1 Glioma Cell Line Tumor CDC2 Protein Kinase Genetics medicine Humans Viability assay Cyclin B1 Molecular Biology Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation PI3K/Akt Chemistry apoptosis Articles Cell cycle Saponins medicine.disease Antineoplastic Agents Phytogenic Triterpenes G2 Phase Cell Cycle Checkpoints 030104 developmental biology medicine.anatomical_structure Oncology Proto-Oncogene Proteins c-bcl-2 Apoptosis 030220 oncology & carcinogenesis Astrocytes Cancer research Molecular Medicine cell cycle Signal transduction Proto-Oncogene Proteins c-akt
Zdroj:	Molecular Medicine Reports
ISSN:	1791-3004 1791-2997
Popis:	Tubeimoside-1 (TBMS1), a traditional Chinese herb extracted from Bolbostemma paniculatum (Maxim.), induces apoptosis in a number of human cancer cell lines. TBMS1 has been reported to induce apoptosis in human glioma cells, however the mechanism remains to be elucidated. The present study explored TBMS1‑induced PI3K/Akt‑related pathways in human glioma cells. The human glioma U251 and the human astrocyte (HA) cell lines were treated with various concentrations of TBMS1. MTT assays were conducted to analyze cell viability. Cell cycle distribution and the rate of apoptosis were assessed using flow cytometry. BrdU incorporation and Hoechst 33342 staining were performed to analyze the cell cycle and apoptosis, respectively. Western blotting was performed to investigate protein expression levels. The results demonstrated that TBMS1 reduced cell viability in human glioma cells U251 by suppressing Akt phosphorylation. Subsequently, TBMS1 inhibited DNA synthesis and induced G2/M phase arrest by targeting the PI3K/Akt/p21 and the cyclin‑dependent kinase 1/cyclin B1 signaling cascades. In addition, TBMS1 triggered apoptosis via the PI3K/Akt‑mediated Bcl‑2 signaling pathway. These results demonstrated that TBMS1 prevented the progression of gliomas via the PI3K/Akt‑dependent pathway, which provided a theoretical basis for in vivo studies to use TBMS1 as potential therapy for the prevention of cancer.
Databáze:	OpenAIRE
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