Facile rhenium–peptide conjugate synthesis using a one-pot derived Re(CO)3 reagent
| Autor: | Christopher J. Ziegler, Thomas C. Leeper, Richard S. Herrick, Sailaja Paruchuri, Vinay Kondeti, Joel Caporoso, Kullapa Chanawanno |
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| Rok vydání: | 2016 |
| Předmět: |
Cell Survival
Stereochemistry chemistry.chemical_element 010402 general chemistry complex mixtures 01 natural sciences Article Inorganic Chemistry chemistry.chemical_compound Solid-phase synthesis Coordination Complexes Human Umbilical Vein Endothelial Cells Humans Peptide sequence Schiff Bases Solid-Phase Synthesis Techniques chemistry.chemical_classification Schiff base 010405 organic chemistry Rhenium 0104 chemical sciences Amino acid Microscopy Fluorescence chemistry Reagent Peptides Neurotensin Conjugate |
| Zdroj: | Dalton Transactions. 45:4729-4735 |
| ISSN: | 1477-9234 1477-9226 |
| DOI: | 10.1039/c5dt04694g |
| Popis: | We have synthesized two Re(CO)3-modified lysine complexes (1 and 2), where the metal is attached to the amino acid at the Nε position, via a one-pot Schiff base formation reaction. These compounds can be used in the solid phase synthesis of peptides, and to date we have produced four conjugate systems incorporating neurotensin, bombesin, leutenizing hormone releasing hormone, and a nuclear localization sequence. We observed uptake into human umbilical vascular endothelial cells as well as differential uptake depending on peptide sequence identity, as characterized by fluorescence and rhenium elemental analysis. |
| Databáze: | OpenAIRE |
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