IN VITRO FUNCTIONAL PROPERTIES OF THE RAT BLADDER REGENERATED BY THE BLADDER ACELLULAR MATRIX GRAFT
| Autor: | S. E. Dahms, Hans J. Piechota, L. Nunes, Tom F. Lue, Rajvir Dahiya, Emil A. Tanagho |
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| Rok vydání: | 1998 |
| Předmět: |
Homologous
Male Detrusor muscle Pathology medicine.medical_specialty Carbachol Urology medicine.medical_treatment Urinary system Clinical Sciences Urinary Bladder Purinergic Stimulation In Vitro Techniques Article Rats Sprague-Dawley Contractility Cystectomy Receptors Muscarinic Receptors Adrenergic beta medicine Animals Regeneration Transplantation Homologous bladder Transplantation Urinary bladder business.industry graft function Receptors Purinergic in vitro Muscle Smooth Urology & Nephrology Immunohistochemistry Receptors Muscarinic Electric Stimulation Rats Urodynamics medicine.anatomical_structure Adrenergic Muscle beta Female Smooth Sprague-Dawley business medicine.drug |
| Zdroj: | The Journal of Urology The Journal of urology, vol 159, iss 5 |
| ISSN: | 1527-3792 0022-5347 |
| Popis: | Purpose To assess the response of rat urinary bladder regenerated by the homologous bladder acellular matrix graft (BAMG) to in vitro electrical and pharmacologic stimuli. Materials and Methods In Sprague-Dawley rats, partial cystectomy (>50%) was performed, followed by BAMG augmentation cystoplasty. After 4 months, organ bath studies of tissue strips in 10 were used to compare the contractility of the BAMG regenerates and the corresponding host detrusor smooth muscle. Results The BAMG regenerates exhibited contractile activity to electrical field stimulation and a qualitatively identical pattern of response to muscarinic, purinergic, alpha- and beta-adrenergic drug administration and nitric oxide. At 4 months after surgery, the maximum forces of contraction of the BAMG regenerates to carbachol stimulation amounted to close to 80% of the host bladder response. With electrical field stimulation, they equaled 44% and 62% of the host bladder response after 2.5 and 4 months, respectively. Histological and immunohistochemical studies confirmed the presence of receptors for neurotransmitters that these functional in vitro studies implied. Conclusions The present study provides further evidence that augmentation cystoplasty with the BAMG leads to functional regeneration of the rat bladder detrusor smooth muscle. |
| Databáze: | OpenAIRE |
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