MicroRNA-210 Modulates the Cellular Energy Metabolism Shift During H2O2-Induced Oxidative Stress by Repressing ISCU in H9c2 Cardiomyocytes
| Autor: | Xing Yue, Wei Sun, Tianyi Li, Xianjing Song, Junduo Wu, Xiao Yanlong, Yiran Wang, Zhibo Li, Lei Zhao, Yinuo Guan, Yang Lu, Ning Liu, Xin Tian, Youyou Yan, Bin Liu, Jichang Zhang, Longbo Li |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Iron-Sulfur Proteins SIRT3 Physiology Cell Survival Carbohydrate metabolism medicine.disease_cause Cell Line 03 medical and health sciences Western blot microRNA medicine Animals Sirtuins Myocytes Cardiac Electron Transport Complex I biology medicine.diagnostic_test Chemistry Antagomirs Metabolism Hydrogen Peroxide NAD Mitochondria Rats MicroRNAs Oxidative Stress 030104 developmental biology Biochemistry biology.protein NAD+ kinase ISCU Energy Metabolism Oxidative stress |
| Zdroj: | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 43(1) |
| ISSN: | 1421-9778 |
| Popis: | Background/Aims: The myocardial energy metabolism shift is one of the most important pathological features of ischemic heart disease (IHD). Although several microRNAs (miRs) are involved in the regulation of myocardial energy metabolism, their exact effects and underlying mechanisms remain unclear. The aim of this study was to investigate whether microRNA(miR-210) regulates the energy metabolism shift during oxidative stress in H9c2 cardiomyocytes. Methods: Cell survival was analyzed via CCK assay. The energy metabolism shift was detected by lactate assay, ATP assay and RT 2 profiler glucose metabolism PCR array. Protein and mRNA expression levels were determined by western blot and qPCR. We also used kits to detect the activity of Complex I, Sirt3 and the NAD + /NADH ratio. Results: We determined that miR-210 promoted the energy metabolism shift. The iron-sulfur cluster assembly protein (ISCU) was a target of miR-210. Additionally, we detected the activity of complex I and found that miR-210 inhibits mitochondrial respiration. Interestingly, miR-210 may also indirectly regulate SIRT3 by regulating ISCU. Conclusion: Our results confirm that miR-210 is essential and sufficient for modulating the cellular energy metabolism shift during H 2 O 2 -induced oxidative stress in H9c2 cardiomyocytes by targeting ISCU. |
| Databáze: | OpenAIRE |
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