Vascular mechanisms underlying the hypotensive effect of Rumex acetosa

Autor: Shamim Khan, Umme Salma, Hafiz Misbah-ud-Din Qamar, Rahila Qayyum, Abdul Jabbar Shah, Taous Khan
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
calcium channel blocker
Vasodilator Agents
Pharmaceutical Science
Aorta
Thoracic
Blood Pressure
Calcium channel blocker
Pharmacology
Polygonaceae
law.invention
Rats
Sprague-Dawley
0302 clinical medicine
law
Drug Discovery
antihypertensive
biology
no-mediated vasorelaxant
General Medicine
Sprague dawley
Vasodilation
Hypertension
Molecular Medicine
Rabbits
medicine.drug_class
Context (language use)
Nitric Oxide
03 medical and health sciences
medicine
Animals
Rumex
Antihypertensive Agents
Plants
Medicinal
Dose-Response Relationship
Drug
business.industry
Plant Extracts
Methanol
lcsh:RM1-950
biology.organism_classification
Nitric oxide metabolism
Rumex acetosa
Plant Leaves
Disease Models
Animal
lcsh:Therapeutics. Pharmacology
030104 developmental biology
Complementary and alternative medicine
Prostaglandin-Endoperoxide Synthases
Solvents
Calcium Channels
Nitric Oxide Synthase
business
Phytotherapy
030217 neurology & neurosurgery
Zdroj: Pharmaceutical Biology, Vol 56, Iss 1, Pp 225-234 (2018)
ISSN: 1744-5116
Popis: Context: Rumex acetosa L. (Polygonaceae) is well known in traditional medicine for its therapeutic efficacy as an antihypertensive. Objective: The study investigates antihypertensive potential of crude methanol extract (Ra.Cr) and fractions of Rumex acetosa in normotensive and hypertensive rat models and probes the underlying vascular mechanisms. Materials and methods: Ra.Cr and its fractions were tested in vivo on normotensive and hypertensive Sprague-Dawley rats under anaesthesia for blood pressure lowering effect. In vitro experiments on rat and Oryctolagus cuniculus rabbit aortae were employed to probe the underlying vasorelaxant mechanism. Results: In normotensive rats under anaesthesia, Ra.Cr caused fall in MAP (40 mmHg) at 50 mg/kg with % fall of 27.88 ± 4.55. Among the fractions tested, aqueous fraction was more potent at the dose of 50 mg/kg with % fall of 45.63 ± 2.84. In hypertensive rats under similar conditions, extract and fractions showed antihypertensive effect at same doses while aqueous fraction being more potent, exhibited 68.53 ± 4.45% fall in MAP (70 mmHg). In isolated rat aortic rings precontracted with phenylephrine (PE), Ra.Cr and fractions induced endothelium-dependent vasorelaxation, which was partially blocked in presence of l-NAME, indomethacin and atropine. In isolated rabbit aortic rings pre-contracted with PE and K+-(80 mM), Ra.Cr induced vasorelaxation and shifted Ca2+ concentration–response curves to the right and suppressed PE peak formation, similar to verapamil, in Ca2+-free medium. Discussion and conclusions: The data indicate that l-NAME and atropine-sensitive endothelial-derived NO and COX enzyme inhibitors and Ca2+ entry blocking-mediated vasodilator effect of the extract explain its antihypertensive potential.
Databáze: OpenAIRE
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