Manothermosonication as a useful tool for lipid extraction from oleaginous microorganisms

Autor: Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat
Přispěvatelé: Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR PROBIO3 (2010–2020), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
Time Factors
Acoustics and Ultrasonics
Linoleic acid
Sonication
Oleaginous microorganism
Rhodosporidium toruloides
Extraction
Chemical Fractionation
01 natural sciences
law.invention
Inorganic Chemistry
Palmitic acid
Manothermosonication (MTS)
chemistry.chemical_compound
0404 agricultural biotechnology
law
lipid
010608 biotechnology
Ultrasound
Chemical Engineering (miscellaneous)
Environmental Chemistry
Flame ionization detector
Radiology
Nuclear Medicine and imaging
amélioration du rendement
Ustilaginales
lipide
chemistry.chemical_classification
Chromatography
microorganisme
biology
oléagineux
Organic Chemistry
Fatty Acids
Temperature
Fatty acid
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
Lipids
Oleic acid
chemistry
lipids (amino acids
peptides
and proteins)
Gas chromatography
[CHIM.OTHE]Chemical Sciences/Other
extraction chimique
Zdroj: Ultrasonics Sonochemistry
Ultrasonics Sonochemistry, Elsevier, 2017, 37, pp.216-221. ⟨10.1016/j.ultsonch.2017.01.014⟩
ISSN: 1350-4177
Popis: Manothermonication is a recognized and efficient method used for sterilization in food preservation. The synergistic effect of sonication combined with pressure and temperature allows enhancing the cavitation activity. Never employed for extraction, this study is about the transposition of this process as a tool of extraction. In this study, Rhodosporidium toruloides yeast was submitted to extraction by four modes of sonication, with a temperature ranged from 20 to 55°C and a pressure between 1 to 2bars. The lipids extraction yields were compared to the conventional maceration. Microbial oils obtained from both processes were analyzed and quantified by HPTLC (High Performance Thin-Layer Chromatography) and GC-FID (Gas Chromatography with flame ionization detector) after transesterification of lipids. Manothermosonication (30min, 2bars, 55°C) permits to enhance of approximately 20% the extraction yield of lipids to compared to conventional maceration. The fatty acid profiles of each pretreatment and extraction by US, MS, TS and MTS do not affect the fatty acid profiles of yeast (majority of oleic acid (C18:1n9), linoleic acid (C18:2n6) and palmitic acid (C16:0)). Manothermosonication technique shows a great potential for lipid extraction from oleaginous microorganisms.
Databáze: OpenAIRE
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