Quantification of Legionella DNA certified reference material by digital droplet PCR

Autor:	Noémie Fessy, Astrid Cariou, Frédéric Pastori, Maud Baume, Adélaïde Leveau, Sophie Pierre, Sophie Jarraud
Rok vydání:	2019
Předmět:	DNA Bacterial Microbiology (medical) 0303 health sciences Chromatography biology 030306 microbiology Legionella Chemistry Stability study Absolute quantification DNA Fragmentation Reference Standards Real-Time Polymerase Chain Reaction biology.organism_classification Microbiology respiratory tract diseases 03 medical and health sciences chemistry.chemical_compound Certified reference materials Real-time polymerase chain reaction Molecular Biology Digital droplet pcr DNA 030304 developmental biology
Zdroj:	Journal of Microbiological Methods. 157:50-53
ISSN:	0167-7012
DOI:	10.1016/j.mimet.2018.12.019
Popis:	A value was assigned in 2009 to the Legionella DNA Certified Reference Material, and the stability study conducted using quantitative PCR found a low level of degradation. Herein, the Digital Droplet PCR method for Legionella DNA was qualified and used to provide absolute quantification of the CRM.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96df6364632a1b6c0c24106a483aab42 https://doi.org/10.1016/j.mimet.2018.12.019 Zobrazit plný text záznamu Full Text from ScienceDirect