Efficient purification of small unsaturated oligoglucuronides by reversed-phase chromatography
| Autor: | Philippe Michaud, Emmanuel Petit, Bernard Courtois, Josiane Courtois, Marie-Laure Tavernier, Anne Wadouachi, David Lesur |
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| Rok vydání: | 2007 |
| Předmět: |
Clinical Biochemistry
Size-exclusion chromatography Glucuronates Uronic acid Biochemistry Analytical Chemistry chemistry.chemical_compound Countercurrent chromatography Column chromatography Trifluoroacetic acid Anion Exchange Resins Chromatography High Pressure Liquid Polysaccharide-Lyases Isocratic elution Chromatography Chemistry Cell Biology General Medicine Reversed-phase chromatography Chromatography Ion Exchange Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Chromatography Gel Spectrophotometry Ultraviolet Ion Exchange Resins Chromatography column Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 863(2) |
| ISSN: | 1570-0232 |
| Popis: | Ion-exchange chromatography has been applied to purification of unsaturated oligoglucuronans. After an isocratic elution on a strong anion-exchange column, the collected fractions were desalted by low pressure size exclusion chromatography. However, this efficient separation was limited by the time required to desalt. So, we developed a reversed-phase chromatography method using back ionization of oligomers. Two C18 columns were tested with trifluoroacetic acid (TFA 0.7%) as eluent. Different selectivities and column stabilities were observed in this acidic condition. The scale up for semi-preparative applications enabled us to recover pure unsaturated oligoglucuronans without desalting step. |
| Databáze: | OpenAIRE |
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