Immunostimulatory effects of pig seminal proteins on pig lymphocytes
| Autor: | W. I. Li, C. K. Smith, Simon C. M. Kwok, L. S. Leshin, S. M. P. Raj, R. R. Kraeling |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Embryology medicine.medical_specialty Swine Lymphocyte chemical and pharmacologic phenomena Semen Lymphocyte proliferation Seminal Vesicle Secretory Proteins Andrology Endocrinology Antigen Internal medicine medicine Concanavalin A Animals Lymphocytes Phytohemagglutinins Cells Cultured Phytohaemagglutinin Glycoproteins biology Dose-Response Relationship Drug Pokeweed mitogen Obstetrics and Gynecology Cell Biology Mixed lymphocyte reaction eye diseases medicine.anatomical_structure Reproductive Medicine Pokeweed Mitogens biology.protein Female Cell Division |
| Zdroj: | Journal of reproduction and fertility. 114(1) |
| ISSN: | 0022-4251 |
| Popis: | Pig seminal proteins PSP-I and PSP-II are major protein components of boars' ejaculate and are present as heterodimers (PSP-dimer) in seminal plasma. These proteins were examined for their ability to modulate pig lymphocyte activity in vitro in mitogen-induced lymphocyte proliferation assays and in one-way mixed lymphocyte reactions. Pig lymphocytes were cultured with phytohaemagglutinin, concanavalin A, or pokeweed mitogen (PWM) in the presence or absence of pig seminal proteins and the amount of cellular [ 3 H]thymidine was used as an indication of proliferation. In the absence of mitogens, none of the three pig seminal proteins affected lymphocyte proliferation suggesting that these proteins are not antigenic or mitogenic. PSP-dimer enhanced lymphocyte proliferation induced by PWM (156-227%, P < 0.05) in a concentration-dependent manner, but had no effect on phytohaemagglutinin- or concanavalin A-induced proliferation. PSP-I enhanced (127-185%, P < 0.05) phytohaemagglutinin-induced proliferation. PSP-II augmented (130-240%, P < 0.05) lymphocyte proliferation induced by concanavalin A and PWM. Lymphocytes from gilts were significantly more responsive to concanavalin A- and PWM-induced lymphocyte proliferation in the presence of PSP-I compared with boars (concanavalin A: gilts 131%, boars 91%; PWM: gilts 188%, boars 134%; P < 0.05). In the mixed lymphocyte reaction, pretreating stimulating cells with increasing concentrations of PSP-I or PSP-II elicited a 400% concentration-dependent increase (P |
| Databáze: | OpenAIRE |
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